rat collagen type iii  (Elabscience Biotechnology)

Journal: Journal of Cosmetic Dermatology

Article Title: ACE2 Inhibits Dermal Regeneration Through Ang II in Tissue Expansion

doi: 10.1111/jocd.16767

Figure Lengend Snippet: ACE2 inhibitor MLN‐4760 elevated the amount of collagens in the expanded rat skin. (a) TGF‐β mRNA expressions in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (b) COL I mRNA expressions in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (c) COL III mRNA expression in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (d) Protein levels of TGF‐β, COL I, and COL III in DMSO‐treated and MLN‐760‐treated skins detected by Western blot. (e) Quantification of the TGF‐β protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). (f) Quantification of the COL I protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). (g) Quantification of the COL III protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). * p < 0.05, and ** p < 0.01.

Article Snippet: The membranes were immersed in a blocking solution for 1 h. The membranes were then immersed at least 12 h at 4°C °C with the subsequent primary antibodies: rabbit anti‐rat ACE2 (1:1000, Abcam, United Kingdom), rabbit anti‐rat COL I (1:2000, Proteintech, China), rabbit anti‐rat COL III (1:1000, Proteintech, China), and rabbit anti‐rat TGF‐β (1:1000, Proteintech, China).

Techniques: Expressing, Western Blot

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/ijn.s466312

Figure Lengend Snippet: Figure 4 RCDs/UA@Lipo alleviated H2O2-induced oxidative stress and TGF-β1 induced fibrosis-specific markers separately. (A) Immunofluorescence staining images of intracellular ROS and superoxide detected by DCFH-DA probe. (B) mitochondrial membrane potential (ΔΨm) detected by JC-1 probe. (C) tendon fibrosis markers (COL I, COL III, α-SMA) of NIH3T3. (D–H) Semiquantitative analysis of the relative fluorescent intensity of (A–C) (n = 3). (D and E) Model group is H2O2 group, (F–H) Model group is TGF-β1 group. Data are presented as mean ± SD; comparisons between the groups were performed one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Evaluation of Anti-Fibrotic Effects of the Biomaterials by Immunofluorescent Staining at Six Weeks The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Membrane

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/ijn.s466312

Figure Lengend Snippet: Figure 6 RCDs/UA@Lipo-HAMA reduced adhesion of injured tendons at macroscopic and histological levels at various time points post-injury. (A) Schematic diagram of surgical procedures of the ATI. (B) Gross view of ATI. (C)Representative images of immunofluorescence staining of tendon antioxidant (Nrf-2, HO-1) and anti-inflammatory markers (CD68, iNOS) at 2 weeks post-injury (n = 3). (D) Representative images of immunofluorescence staining of tendon antifibrosis (COL III, α-SMA) at 6 weeks post- injury (n = 3). (E) Representative images of immunohistochemical staining of tendon markers (Vimentin, MMP2, α-SMA) antifibrosis at 6 weeks post-injury (n = 4). (F–N) Semiquantitative analysis of expression level of tendon marker of (C–E), respectively. Data are presented as mean ± SD; comparisons between the groups were performed by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: Evaluation of Anti-Fibrotic Effects of the Biomaterials by Immunofluorescent Staining at Six Weeks The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, Marker

Journal: International Journal of Molecular Sciences

Article Title: Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production

doi: 10.3390/ijms222313089

Figure Lengend Snippet: Col I/III production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by ELISA. ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Collagen I and collagen III were assessed using Rat Collagen Type I ELISA kit (Cusabio, Wuhan, China, #CSB-E08084r) and Rat Collagen Type III ELISA kit (Cusabio, Wuhan, China, #CSB-E07924r) according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Standard Deviation

Journal: bioRxiv

Article Title: Effect of therapeutic ultrasound on the mechanical and biological properties of fibroblasts

doi: 10.1101/2021.11.22.469508

Figure Lengend Snippet: Protein concentration in cell culture supernatants of ligament fibroblasts. Values were measured on the 6 th day and 10 th day after stimulation period for type I collagen, type III collagen, and fibronectin.

Article Snippet: We used rat collagen type I (E-EL-R0233), rat collagen type III (E-EL-R0235), and rat fibronectin (E-EL-R0578) Elabscience® ELISA kits.

Techniques: Protein Concentration, Cell Culture